Supplementary Figure 5: Detailed results of the social interaction test in control and susceptible mice treated with either acute or chronic idazoxan.
From: Resilience to chronic stress is mediated by noradrenergic regulation of dopamine neurons
(a) In susceptible mice, the time spent (s) in the interaction zone with target was significantly lower than the time spent (s) with no target in both the NaCl (n=10) and the idazoxan-treated (n=8) groups (Target effect: post-defeat F(1,16)=24.44, p=0.00015, post-hoc ***p<0.00019 and acute treatment F(1,16)=5.9, p=0.027, post-hoc *p=0.039). However, no differences between groups were observed during the two sessions. (b) After 1 week of treatment, the correlation between the interaction score (Target/NoTarget) and the time spent in the interaction zone (s) with the target showed that 10% (1/10) of the NaCl-treated mice spent more than 60s interacting with the target compared with 75% (6/8) of the idazoxan-treated mice. (c) In control animals, either acute or chronic idazoxan (2 mg/kg, i.p.) treatment had no effect on the time (s) spent in the interaction zone with target (F(2,42)=0.17, p=0.85, left). The time spent in the interaction zone (s) with target was either increased (post-defeat, ***p<0.001) or similar (Acute and 1 week) than the time spent in the interaction zone (s) with no target in both the control NaCl (n=11) and idazoxan-treated (n=12) groups. However, no differences between control groups were observed during the two sessions and the three timepoints.
