Supplementary Figure 4: Relation of dmPFC and BLA 4-Hz oscillations with freezing, immobility and motor behavior.

(a) Representative spectrograms of BLA LFPs recorded during Habituation. White lines on the spectrogram indicate immobility periods. (b) Correlation coefficient between the power envelope of the 4 Hz oscillations (2–6 Hz bandpass) recorded in the BLA and immobility/freezing behaviour during Habituation (Hab.) and Retrieval sessions (n = 13 mice, paired t-test, BLA: t(12) = -4.871, *** P < 0.001). (c) Receiver operating characteristics (ROC) analyses performed on Naïve Bayes (NBC) and Support Vector Machine (SVM) classifiers trained on 4 Hz signal to noise ratio (SNR) of the BLA during freezing episodes (see Methods). Inset: averaged area under the curve for NBC and SVM classifiers compared to corresponding shuffled data. (d) Percentage of accuracy of the NBC and SVM classifiers to predict freezing behaviour based on BLA 4 Hz SNR (see Methods). (e) Correlation analysis performed between freezing and 4 Hz episodes for the BLA (n = 13 mice; BLA: Pearson's r coefficient = 0.01, P = 0.974). (f) Location of recording sites in the lateral (lPAG) and ventrolateral periaqueductal grey (vlPAG) (n = 5 mice). (g) Left, Averaged power spectrum of PAG LFPs recorded during Retrieval for freezing and no freezing periods (n = 5 mice). Right, Averaged PAG 2-6 Hz power during Retrieval for freezing and no freezing periods (n = 5 mice, paired t-test, t(4) = 0.098, P = 0.926). Shaded areas and error bars: mean ± s.e.m. Spectral power in log scale. a.u.: arbitrary units. For the representative example in (a), similar results were obtained for the 13 animals used in these experiments.