Supplementary Figure 9: Oscillatory activity of dmPFC and BLA cells.

(a) Representative auto-correlogram from a dmPFC putative principal neuron on which a Gabor function was fitted to evaluate its oscillatory activity (see Methods section). (b, c) Percentage of dmPFC (b) and BLA (c) putative excitatory principal neurons and inhibitory interneurons that were phase-locked to 4 Hz and displaying 4 Hz oscillatory activity. (d) Left, Representative post-stimulus time histogram (PSTH) during optogenetic stimulation of an identified dmPFC parvalbumin-expressing (PV) interneuron. Blue shaded area corresponds to the light stimulation period. Right, Representative spiking activity of an identified PV interneuron in relation to dmPFC LFPs recorded during freezing behaviour. (e) Representative (blue bars) and averaged (red dots) phase-histogram of optogenetically identified dmPFC PV interneurons (n = 18 PV cells collected in 13 mice). (f) Percentage of optogenetically identified dmPFC PV interneurons phase locked to dmPFC 4 Hz oscillations (13 out of 18 PV cells (72%)). (g) Circular distribution of the 4 Hz oscillation preferred phase for populations of optogenetically identified dmPFC PV interneurons phase-locked to 4 Hz during freezing behaviour. (h) Representative auto-correlogram from optogenetically identified dmPFC PV interneuron on which a Gabor function was fitted to evaluate its oscillatory activity (see Methods section). (i) Corresponding representative correlation analysis performed between spike counts and Gabor function amplitude (Pearson's r coefficient = 0.79, P < 0.001). (j) Percentage of optogenetically identified dmPFC PV interneurons phase-locked to 4 Hz displaying 4 Hz oscillatory activity (11 out of 13 PV cells (84.6%)). For the representative traces in (a, d-e, and h-i), similar results were obtained for the animals used in these experiments (13 mice in a, and 13 mice in d-e and h-i).