Supplementary Figure 4: Comparison of FFN200 and FFN102 release in the dorsal striatum.
(a) Background intensity presented as mean percentage of Fi ± SEM for both probes (n=9 and 8 slices from different mice for FFN200 and FFN102, respectively, for all panels in this figure). (b) Scatter plot of the percentage of FFN200 and FFN102 destaining puncta in response to 15 Hz stimuli in each independent experiment including mean ± SEM (n.s- not significantly different, p=0.1269, two-tailed unpaired t test). As a control, the percentage of FFN102 puncta selected as “destainers” in unstimulated slices was 5.1 ± 1.6% (n=5; not depicted). (c) FFN200 and FFN102 puncta intensity (background- and baseline rundown-corrected) over time, normalized to ΔF, the fluorescence change between the last point of the baseline (100%) and the average of the last three data points of stimulation (0%) ± SEM, to facilitate comparison between FFN200 and FFN102 curves (*p < 0.05, two-tailed unpaired t test; p=0.0370). (d) Cumulative distribution of t1/2s of destaining for FFN200 and FFN102 (147 and 175 puncta from nine and eight slices from different mice for FFN200 and FFN102, respectively).
