Supplementary Figure 3: OL number and myelination recovery in adult Chd7 mutant mice
From: Chd7 cooperates with Sox10 and regulates the onset of CNS myelination and remyelination
(a) Immunolabeling of CC1 on transverse spinal cord sections from Chd7cKO and control mice at postnatal days 4, 7, 15 and 60. Scale bar, 100 μm.
(b) Quantification of CC1+ cells density (top) and CC1+ cell proportion among total Sox10+ cells (bottom) in the white matter region of spinal cords from Chd7cKO and control mice at P1, P4, P7, P15 and P60 (mean ± SEM, n = 3; top panels: for P1, * p = 0.03, t = 3.89; for P4, ** p = 0.0029, t = 6.49; for P7, * p = 0.012, t = 4.36; for P14, * p = 0.045, t = 2.87; for P60, p = 0.71, t = 0.39; n, number of animals; unpaired two-tailed Student’s t test; bottom panels, F(1, 19) = 56.75; Two-way ANOVA test).
(c) Representative electron microscopy images for P60 spinal cord of control and Chd7cKO mice. Scale bar, 2 μm.
(d) Quantification of myelin thickness determined by g-ratio. n = 3 animals of each genotype. No statistical difference of g-ratio between control and mutants.
