Supplementary Figure 2: Cortical white matter volume and OL numbers are reduced in Chd7 mutant mice
From: Chd7 cooperates with Sox10 and regulates the onset of CNS myelination and remyelination
(a) RNA in situ hybridization for Olig1 in P4 and P15 coronal brain (Br) sections from control and Chd7cKO mice. Scale bar, 100 μm.
(b) Quantification of Olig1 ISH density in the corpus callosum of control and Chd7cKO mice at P4 and P15 (mean± SEM; for P4, n = 2, ** p = 0.007, t = 11.359; for P15, n = 3, ** p = 0.0027, t = 6.575; n, number of animals; unpaired two-tailed Student’s t test).
(c) Immunostaining for Sox10 (upper panels) and Olig2 (bottom panels) in the corpus callosum of control and Chd7cKO mice at P9 and P15. Scale bar, 100 μm.
(d) Quantification of Sox10+ and Olig2+ cell density in the control and Chd7cKO corpus callosum at P9 and P15 (mean± SEM; top panels for Sox10: for P9, n = 3, * p = 0.017, t = 3.93; for P15, n = 4, ** p = 0.0027, t = 4.877; bottom panels for Olig2: for P9, n = 3, * p = 0.02, t = 3.74; for P15, n = 4, * p = 0.014, t = 3.38; n, numbers of animals; unpaired two-tailed Student’s t test).
(e) Examples of serial brain sections immunolabeled with MBP from Chd7cKO and control mice at P9. Nuclei are counterstained with DAPI. Scale bar, 1 mm.
