Supplementary Figure 9: Application of Magneto1.0 to zebrafish behavior in vivo.
From: Genetically targeted magnetic control of the nervous system
(a) Schematic of trans cardiac myosin light chain 2 (cmcl2)::GFP element and its expression in 24 hpf zebrafish embryos for positive transgenic selection. n>100 fish examined. (b) Schematic of Magneto1.0 construct used: Tol2: Tol2 transposon sites; ß-Actin: promoter; IRES: internal ribosomal entry site; nls-EGFP: nuclear localized enhanced GFP. (c) Quantification of the number of coils in WT (uninjected) and ß-actin::Magneto1.0 expressing 24 hpf zebrafish embryos in response to magnetic stimulation. n=43 WT, n=25 ß-actin::Magneto1.0 fish. Statistics determined by Chi-squared analysis, (Chi23=36.51, p<0.0001). (d) Quantification of coiling rate in WT (uninjected) and ß-actin::Magneto1.0 expressing zebrafish. Replicates (number of individual fish) shown in columns. Statistics determined by one-way ANOVA, Bonferroni post-test, (F3,64=3.89, p=0.0129). ***p<0.001, *p<0.05. Data are shown as mean±SEM.
