Supplementary Figure 3: In vitro calcium imaging using Magneto1.0.
From: Genetically targeted magnetic control of the nervous system
(a) Mammalian expression vector schematic of Magneto1.0. (b-g) Representative images of HEK293 cells used for in vitro magnetic stimulation Fluo-4 calcium imaging. (h) Quantification of relative calcium fluorescence in response to magnetic stimulation of mCherry+ cells. Replicates are shown as individual coverslips equaling n=6 (TRPV4/ferritin), n=8 (Magneto1.0), and n=6 (Magneto1.0+RR). Total cells analyzed for each condition are n=545 (TRPV4/ferritin), n=565 (Magneto1.0), and n=437 (Magneto1.0+RR). One-way ANOVA, Bonferroni post-test, (F2,17=7.509, p=0.0046). (i) Representative images of temporal association between calcium fluorescence and magnetic field pulses in an individual Magneto1.0-expressing cell (arrow). Field was pulsed for alternating 10 second periods of on/off. *p<0.05. Data are shown as mean±SEM.
