Supplementary Figure 3: Interneuron diversity in the LEC.

Confocal images of sections from a GAD67EGFP mouse brain immunostained with antibodies against (a) PV, (b) SOM, (c) CR, (d) CB, (e) RE and (f) 5-HT3AR with corresponding graphs showing the distribution in LIIa and LIIb. Counting was performed in 3-4 hemispheres from 2 mice. For experiments as illustrated in f, 5-HT3-EGFP mice were used. Furthermore, RE and CB stainings were employed with the purpose to visualize the border between LIIa and LIIb. Scale bar, 100 μm. Abbreviations: CR, Calretinin; EGFP, enhanced green fluorescent protein; L, layer; PV, Parvalbumin; SOM, Somatostatin; 5-HT3AR, ionotropic serotonin receptor 5-HT3A.