Supplementary Figure 10: Properties of odor-evoked calcium responses of RE⁺, CB⁺ and GABAergic neurons (top to bottom row).

(a) Overlay of all trial-averaged GCaMP6 fluorescence traces during odor stimulation, sorted into significant positive responses ('Pos'), non-significant traces ('NS'), and significant negative responses ('Neg'). Significance was evaluated within the 4-s odor stimulation period. Note that for RE⁺ and CB⁺ neurons some NS and Neg traces display late fluorescence changes (number of responses for RE⁺: 723 Pos, 2021 NS, 340 Neg; CB⁺: 871 Pos, 1413 NS, 158 Neg; GAD: 378 Pos, 720 NS, 66 Neg). (b) Group-wise average of the respective Pos, NS, and Neg traces shown in a. (c) Histograms of fluorescence peaks during odor stimulation for significant positive and negative responses for the different cell types. Note that the tail of responses larger than 400% is truncated (cut events: 11 RE⁺, 6 CB⁺, 2 GAD). (d) Histograms of estimated onset latencies for significant fluorescence responses for the different cell types (peak position/half-width: RE⁺ 0.4 s/0.7 s, CB⁺ 0.4 s/0.6 s, GAD 0.3 s/0.5 s). (e) Histograms of fluorescence response durations (full width at half-maximum) for the different cell types (evaluated in a 19-s time window following stimulus onset). Note the bimodality of the distributions, indicating the fraction of neurons displaying a prolonged, late response component.