Supplementary Figure 5: Zeb2 regulates genes involved in the regulation of radial sorting
From: Zeb2 recruits HDAC–NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination
(a) Differential expression of selected genes involved in radial sorting regulation was detected based on transcriptome profiling of P7 control and Zeb2 cKO sciatic nerves. The table lists selected genes reported to inloxPuence radial sorting, their fold change in Zeb2 cKO sciatic nerves relative to control nerves and the sorting defects in corresponding mutants. Downregulated and upregulated genes are highlighted in red and blue, respectively. (b) qRT-PCR analysis of independently prepared P7 control and Zeb2 cKO sciatic nerves confirms the differential gene expression. Data are presented as mean ± S.E.M (**p < 0.01; ***p < 0.001; n = 3 independent experiments, P(Cdc42) = 0.0057 t = 5.402 df = 4; P(Dag1) = 0.0631 t = 2.553 df = 4; P(Itga6) = 0.001 t = 8.511 df = 4; P(Itga7) = 0.0004 t = 11.28 df = 4; P(Lama2) = 0.0007 t = 9.289 df = 4; Two-tailed unpaired Student’s t-test).
