Supplementary Figure 6: Controls for stimulation of cholinergic axons in the VTA.

(a) Following injection of AAV2-DIO-EF1a-eYFP into the PPN/LDT in ChAT::cre rats, individual TH+ neurons (n = 5 neurons) were recorded in vivo during blue light delivery and subsequently labeled with neurobiotin (b). The same protocol was followed as that used in figures 1 and 2. No change in firing was observed in neurons transduced with YFP only. Scale bar: 20 µm.