Supplementary Figure 8: Genotyping of iPSCs and NPCs derived from a MECP2R306C RTT patient and corresponding isogenic control lines generated by CRISPR/Cas9 gene editing.
From: Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior
(a) - (d) Chromatogram view of MECP2R306C locus following sequencing of genomic DNA (gDNA) and complementary DNA (cDNA) from iPSC and NPC lines as indicated. Sequences were analyzed for MECP2R306C iPSCs (a, b), line 4 and 14 MECP2R306C NPCs (c), line 6 and 36 iPSCs, and line 6 and 36 NPCs (d). Purple inset indicates the nucleotide position of the R306C mutation. Green inset indicates the nucleotide position of the corrected R306C mutation following CRISPR/Cas9 gene editing. Red insets indicate intentional introduction of heterozygous silent mutations following CRISPR/Cas9 gene editing.
