Supplementary Figure 3: Hdac3 cKO mice exhibit deficits in sociability and hippocampal-dependent learning.

(a) Time spent in chambers during 10 minute habituation phase of the sociability task (Fig. 1d) for control and Hdac3 cKO mice. Control; one-way ANOVA, F_{(2, 24)} = 0.1687, P = 0.8457; Bonferroni post hoc, Left versus Center, P > 0.99; Left versus Right, P > 0.99; Center versus Right, P > 0.99; n = 9 mice. Hdac3 cKO; one-way ANOVA, F_{(2, 24)} = 2.793, P = 0.0812; Bonferroni post hoc, Left versus Center, P = 0.4200; Left versus Right, P > 0.99; Center versus Right, P = 0.0864; n = 9, 9 mice. (b) Total time spent with both objects (s) during the object location memory task (Fig. 1e); two-tailed t test; t₍₂₆₎ = 3.883; *** P = 0.0006; n = 16, 12 mice. (c) The average swim speed (m s⁻¹) of the mice was recorded during the training days (day 1 – 7) and on the probe trial day (day 8) of the MWM task. Two-way ANOVA; Genotype, F_(1,133) = 9.530, ** P =0.0025; Day, F_(6,133) = 3.050, ** P = 0.0079; Bonferroni post hoc; day 1, P > 0.99; day 2, P > 0.99; day 3, P > 0.99; day 4, P > 0.99; day 5, P = 0.0677; day 6, P > 0.99; day 7, P = 0.2703; n = 10, 11 mice. (d) Mean velocity (cm s⁻¹) measured during the habituation and the e-stimulation phases on the training day of fear-conditioned learning; Control: two-tailed t test; t₍₂₀₎ = 15.13; **** P < 0.0001; Hdac3 cKO: t₍₂₀₎ = 17.92; **** P < 0.0001; n = 11, 11 mice. (e) Activity suppression of control and Hdac3 cKO mice during testing of contextual memory; two-tailed t test; t₍₂₀₎ = 3.973; *** P = 0.0007; n = 11, 11 mice. (f) Activity suppression of control and Hdac3 cKO mice during testing of cued (tone) memory; two-tailed t test; t₍₂₀₎ = 5.069; **** P < 0.0001; n = 11, 11 mice. c, report mean value ± s.e.m. a, b, d - f, report median, 25^th and 75^th percentile, min and max value.