Supplementary Figure 4: Transcriptional regulation in the Hdac3 cKO.
From: Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior
(a) qRT-PCR analysis of RNA extracted from the CA1 region of control and Hdac3 cKO mice. Primers designed within exon 10 (non-floxed) and exon 11 (floxed). Two-tailed t test; t(6) = 7.390, *** P = 0.0003; n = 4, 4 mice. Values are mean ± s.e.m. (b) RT-PCR analysis of RNA extracted from the CA1 region of control and Hdac3 cKO mice. Primers designed within exons 10 and 15, flanking floxed exons 11 – 14, n = 2, 2 mice. (c) log2fold change analysis of Hdac3 exon read counts between control and Hdac3 cKO mice following RNA-sequencing of the CA1 region of the hippocampus; n = 2, 2 mice. (d) UCSC genome browser visualization of RNA-seq coverage at the Hdac3 locus for control (blue) and Hdac3 cKO (red). The y-axis is number of reads mapped.
