Supplementary Figure 6: Hippocampal molecular changes either in untrained (naïve) conditions or following IA training at PN17 or PN24.

(a) Representative examples and densitometric western blot analyses of dorsal hippocampal total extracts from naïve rats euthanized at PN17, PN24 or PN80 (adult) (n=8, 8, 8). Data are expressed as mean percentage ± s.e.m. of adult naïve rats [One–way ANOVA followed by Newman-Keuls Multiple Comparison Test, TrkB F(2,21)= 0.6080, P=0.5537; GluN1 F(2,21)= 0.1954, P=0.8240, 3 independent experiments]. (b-c) Representative examples and densitometric western blot analyses of dHC total extracts from rats trained in IA at (b) PN17 or (c) PN24, and euthanized 30min, 9h, 24h or 48h after training (n=6-10/group). To account for developmental differences, two groups of naïve were used [(b) PN17 and PN19 or (c) PN24 and PN26]. Data are expressed as mean percentage ± s.e.m. of (b) PN17 [n= 8, 6, 10, 7, 6, 6, One–way ANOVA followed by Dunnett's Multiple Comparison Test, TrkB F(3,27)= 0.1618, P= 0.9211; GluN1 F(3,27)= 0.08967, P= 0.9650; 3 independent experiments] or (c) PN24 naïve rats [n=8, 6, 6, 7, 7, 8, One–way ANOVA followed by Dunnett's Multiple Comparison Test, TrkB F(3,23)= 0.06099, P= 0.9798; GluN1 F(3,23)= 0.2067, P= 0.8907; 3 independent experiments]. The numeric values are reported in Supplementary Table 3.