Supplementary Figure 2: Properties of spinal responses to cooling.
From: The coding of cutaneous temperature in the spinal cord
(a) The percentage of total neurons at different depths below the surface that are activated by cooling to 29 °C (yellow) and 5 °C (blue) (25 μm, n = 2 mice; 35 μm, n = 5 mice; 45 μm, n = 5 mice; 55 μm, n = 5 mice; 65 μm, n = 4 mice; 75 μm, n = 3 mice; 85 μm, n = 2 mice.) (b) Heat maps (middle) and example traces (bottom) of neuronal responses to cooling (top) in the spinal cord before (left) and after (right) application of NBQX (an AMPA and kainite receptors antagonist, 60 μM) and APV (an NMDA receptor antagonist, 150 μM). Scale bars, 10 s and 10% ΔF/F. (c) Top: An example FOV illustrating OGB (green) labeling in the spinal cord of a Mgfap-cre; Ai14 (cre-dependent tdTomato, red) mouse. Scale bar, 100 μm. Bottom: example calcium traces of cold response from astrocytes (red) and neurons (green). Scale bars, 10 s and 10% ΔF/F.
