Supplementary Figure 5: Diphtheria toxin (DT) treatments of the TRPM8-DTR-GFP and TRPV1-DTR-GFP mice.
From: The coding of cutaneous temperature in the spinal cord
(a) The expression of TRPM8 (in situ hybridization) and TRPV1 (immunofluorescence) in the DRGs of wild type, TRPM8- and TRPV1-DTR mice after diphtheria toxin treatment. Similar observations were made in all mice (number specified below). Scale bar, 100 μm. (b) Quantification of a (Left: n = 2 mice for WT, n = 5 mice for TRPM8-DTR and n = 4 mice for TRPV1-DTR, right: n = 4 mice per genotype). (c) The expression of GFP (immunofluorescence) in the DRGs of saline- or DT-treated TRPM8- and TRPV1-DTR-GFP mice. Similar observations were made in all mice (number specified below). Scale bar, 100 μm. (d) Quantification of c (TRPM8-DTR-GFP: n = 2 mice per treatment group, TRPV1-DTR-GFP: n = 4 mice per treatment group).
