Supplementary Figure 3: Enpp6 expression is oligodendrocyte-specific.
From: Rapid production of new oligodendrocytes is required in the earliest stages of motor-skill learning
Forebrain sections of P90 mice (caged without wheels) were analyzed by ISH for Enpp6 mRNA followed by immuno-fluorescence labeling for stage-specific markers of the oligodendrocyte lineage (Sox10, Olig2, CC1). Alternatively, sections were analyzed by double fluorescence ISH for Enpp6 and Pdgfra. (a,b) All Enpp6+ cells were also Olig2+ and Sox10+. (c) No Enpp6+ cells were Pdgfra+ OPs. (d) Most Enpp6+ cells were CC1+ mature or maturing oligodendrocytes (Motor cortex, 98.2 ± 1.8%; Subcortical white matter, 86.9% ± 3.8%) (Supplementary Table 1). Images are representative of >3 similar experiments. Scale bar, 25 μm.
