Supplementary Figure 2: Two populations of high- and low-expressing Enpp6+ cells in vivo.
From: Rapid production of new oligodendrocytes is required in the earliest stages of motor-skill learning
(a,b) Coronal sections of P60 mouse forebrains were incubated with an Enpp6 ISH probe and the signal developed using the NBT/BCIP method (Methods). (a) At a relatively long development time (150 minutes) two populations of Enpp6-positive cells are detected in the subcortical white matter – a few strongly-labeled large cell bodies (arrows) against a background of more numerous, small, weakly-labeled cells (arrowheads), consistent with the RNA-seq data27 (Fig. 3A) and the likelihood that the strongly-labeled cells are newly-differentiating oligodendrocytes and the weakly-labeled cells more mature, myelinating oligodendrocytes. (b) Reducing the development time to 60 minutes allows the strongly-labeled cells to be visualized preferentially (arrows). Images are representative of >3 similar experiments. Scale bar, 50 μm.
