Supplementary Figure 5: Criteria for bouton identification.
From: Selective synaptic remodeling of amygdalocortical connections associated with fear memory
(a, b) Fluorescent intensity was measured along identified axons. Bright swellings were identified as boutons when the peak intensity was over 3 fold that of the axon shaft (blue dotted line). (c, d) When changing the threshold to 2 fold (green solid line in B), results of bouton counting were not significantly different using the two different criteria. Each data point represents results from one image stack (>100 boutons each).
