Supplementary Figure 3: Rolling responses of larvae ectopically expressing trpA1-A in mdIV neurons exposed to different rates of temperature increase.
From: TRPA1 mediates sensation of the rate of temperature change in Drosophila larvae
(a-i) The fraction of second-instar larvae that rolled (Frolling) as a function of the rate of temperature change (dT/dt). Blue, ppk-GAL4/UAS-trpA1-A;trpA11 (Total number of larvae per experiment ≥ 30. n = 3, 3, 6, 3, 3, 3, 4, 3 and 5 in a, b, c, d, e, f, g, h and i, respectively). Red, UAS-trpA1-A;trpA11 (Total number of larvae per experiment ≥ 30. n = 4, 3, 5, 3, 3, 3, 4, 3 and 5 in a, b, c, d, e, f, g, h and i, respectively). Green, ppk-GAL4;trpA11, only in a, e and i (Total number of larvae per experiment ≥ 30. n = 3, 4 and 4 in a, e and i respectively). The dT/dt are indicated above each plot. The scale bars indicate the seconds required for the temperature to rise by 5 °C. The rolling fraction was defined as Nrolling/Ntotal, where Nrolling was the number of larvae rolling and Ntotal was the total number of larvae. Fpeak was the maximum rolling fraction and Tmiddle was the temperature corresponding to the point when the rolling fraction rose to half the Fpeak. The curves (solid black lines) were fit using a sigmoid function. (j) Fpeak values as a function of dT/dt. Crosses, average Fpeak values in response to the different temperature-change rates. (k) Tmiddle values as a function of dT/dt. Crosses, average Tmiddle values. ppk-GAL4/UAS-trpA1-A;trpA11, one-way ANOVA of Fpeak: F8,24 = 17.81; P = 2.4 x 10-8; one-way ANOVA of Tmiddle: F8,24 = 1.79; P = 0.13; Tukey-Kramer test for statistically significant differences relative to the highest dT/dt (0.5 °C/s): Fpeak: 0.5 °C/s versus. 0.02 °C/s: q24,9 = 8.77; P = 6.3 x 10-5. *P < 0.05, **P < 0.01. Error bars indicate s.e.m.
