Supplementary Figure 11: Apparatus for assaying larval thermal nociception behavior.
From: TRPA1 mediates sensation of the rate of temperature change in Drosophila larvae
The black, plastic outer box (2’ x 2’ x 4’) eliminates external light. We used red (560 nm) LED lights to evenly illuminate the larvae placed on the agarose plate. The camera we used to monitor the larval behaviors was a 2592 x 1944 Monochrome CMOS Camera (GigE). We used a TC-36-25-RS232 temperature controller (TE Technology) to control the temperature changes of the 12” x 12” Peltier plates. We integrated the controller program with the larval tracking software.
