Supplementary Figure 3: Whole-brain distributions of inputs to VIS, SS and AUD.
From: Organization of long-range inputs and outputs of frontal cortex for top-down control
(a) Viral vectors and injection procedure for RV-mediated trans-synaptic retrograde tracing. (b) Fluorescence images of LGd, VP and MG (yellow box in coronal diagram), showing RV-labeled input neurons (red) to VIS, SS and AUD, respectively (scale bar, 100 μm). Red, tdTomato; blue, DAPI. (c-e) RV-labeled neurons detected in all samples of each group (VIS, blue; SS, red; AUD, green; scale bar, 1 mm). White masks indicate injection sites excluded from data analysis. (f) Percentages of retrogradely labeled input neurons in selected cortical and subcortical brain regions (VIS, n = 3 mice; SS, n = 3; AUD, n = 3). Included are cortical areas with > 2% labeling and thalamic structures with > 1% labeling. Each circle represents one mouse. Error bar, ± s.e.m.
