Supplementary Figure 5: Whole-brain distributions of axonal projections from VIS, SS and AUD.
From: Organization of long-range inputs and outputs of frontal cortex for top-down control
(a) Viral vector and injection procedure for tracing axonal projections. (b) Fluorescence images of LGd/LGv, VP and MG (yellow box in coronal diagram), showing the axons (red) from VIS, SS and AUD, respectively (scale bar, 200 μm). Red, mCherry; blue, DAPI. (c-e) Axons detected in all samples of each group (VIS, blue; SS, red; AUD, green; scale bar, 1 mm). White masks indicate injection sites excluded from data analysis. (f) Percentages of labeled axons in cortical and subcortical brain structures (VIS, n = 3 mice; SS, n = 3; AUD, n = 3). Included are cortical areas with > 1% labeling and thalamic structures with > 0.8% labeling. Each circle represents one mouse. Error bar, ±s.e.m.
