Supplementary Figure 7: ACA innervates Pvalb+, Sst+ and Vip+ interneurons and glutamatergic neurons (Camk2α+) in VIS, PTLp and RSP.
From: Organization of long-range inputs and outputs of frontal cortex for top-down control
(a) Viral vectors and injection procedure for RV-mediated transsynaptic retrograde tracing. (b) Upper panel, viral injection sites in VIS (red box in coronal diagram) of Pvalb-Cre, Sst-Cre and Vip-Cre mice (scale bar, 200 μm). Inset, enlarged view of the region in white box showing starter cells (yellow; scale bar, 20 μm). Lower panel, fluorescence images of ACA and MO (red box in coronal diagram) showing RV-labeled input neurons (green) to each subtype of interneurons in VIS (scale bar, 200 μm). Green, EGFP; red, mCherry; blue, DAPI. (c) Similar to (b), for ACA inputs to PV+, SOM+, VIP+ interneurons and glutamatergic neurons in PTLp. (d) Similar to (b), for ACA inputs to Pvalb+, Sst+, Vip+ interneurons and glutamatergic neurons in RSP. (e) Percentages of input neurons in ACA retrogradely labeled from VIS, PTLp and RSP in Camk2α-Cre (yellow), Pvalb-Cre (blue), Sst-Cre (red) and Vip-Cre mice (green). n = 3 mice in each group. Each circle represents one mouse. Error bar, ± s.e.m.
