Supplementary Figure 12: WGA-Cre-labeled ACA→VIS and ACA→SC neurons (EYFP+) largely overlapped with Retrobead-labeled ACA→VIS and ACA→SC neurons, respectively.
From: Organization of long-range inputs and outputs of frontal cortex for top-down control
(a) Viral vectors and retrobead injection procedure for retrograde labeling of ACA→VIS and ACA→SC neurons with EYFP (mediated by WGA-Cre) or red retrobeads. (b) Left, fluorescence images of AAV-EF1α-mCherry-IRES-WGA-Cre and red retrobeads injection site (red) in VIS (upper panel) and SC (lower panel). Middle, retrobeads (red) and WGA-Cre-induced EYFP expression (green) in ACA. Right, enlarged view of the region in white box. White arrowheads, neurons containing both EYFP and retrobeads. Blue, DAPI. Note that most EYFP+ neurons in ACA were also labeled by red retrobeads (VIS injection, 83 ± 4% (s.e.m.), n = 3 mice; SC injection, 80 ± 4%, n = 3 mice).
