Supplementary Figure 15: Whole-brain distributions of inputs to MO→SS neurons.
From: Organization of long-range inputs and outputs of frontal cortex for top-down control
(a) Viral vectors and injection procedure for RV-mediated transsynaptic retrograde tracing from MO→SS neurons. (b) Left, fluorescence images of AAV-EF1α-mCherry-IRES-WGA-Cre injection site in SS (scale bar, 1 mm). Middle, injection site of other AAVs and RV in MO (scale bar, 1 mm). Inset, enlarged view of the region in white box showing AAV/RV infected neurons (green, scale bar, 20 μm). Right, retrogradely labeled neurons (green) in SS (scale bar, 200 μm). Inset, enlarged view of the region in white box (scale bar, 20 μm). Green, EGFP; red, mCherry; blue, DAPI. (c) RV-labeled neurons detected in all MO→SS samples. White masks indicated the injection sites excluded from data analysis (scale bar, 1 mm). (d) Percentages of retrogradely labeled neurons in selected cortical and subcortical brain structures (MO→SS, n = 4 mice). Each circle represents one mouse. Error bar, ±s.e.m. Data for MO inputs are the same as in Figure 3D.
