Supplementary Figure 10: Microglia, astrocytes and vascular endothelia cells in Pten mutant mice
From: A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination
(a-c) Numbers of microglial cells stained for Iba1 (in a) or Mac-3 (in b) are increased in the ML (in a: p=0.0413, t=3.184, d.f.=4; in b: p=0.0295, t=3.315, d.f.=4) but not in the GL (in a: p=0.5484, t=0.912, d.f.=4; in b: p=0.9888, t=0.015, d.f.=4) of Pten mutant mice. Likewise, the area of GFAP+ astrocytes is increased in the mutant ML (p=0.0406, t=2.983, d.f.=4) but not in GL (p=0.141, t=1.831, d.f.=4) (in c). Arrowheads (in a) point to single Iba1+ cells.
(d) The area covered by vascular endothelial cells is increased in the mutant GL compared to controls as quantitated in lobe 5 (p=0.0498, t=2.78, d.f.=4).
n=3 mice each genotype; age: 2.5 months. Data are means ± s.e.m. *p<0.05, two-tailed unpaired Student’s t-test. GL, GC layer; ML, molecular layer. Images (in a and d) are representative of 3 similar experiments.
