Supplementary Figure 11: No signs of gliosis in NEX-CreERT2;PtenloxP/loxP mice
From: A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination
Upon sparse recombination, Nex-CreERT2 ;PtenloxP/loxP mutants show no increase of Iba1+ (in a) or Mac-3+ microglial cells (in b) when compared to controls in ML (in a: p=0.8886, t=0.1523, d.f.=4; in b: p=0,1494, t=1.782, d.f.=4) and GL (in a: p=0.516, t=0.7343, d.f.=4; in b: p=0,7067, t=0.4042, d.f.=4). Also the GFAP+ astroglial area is similar in mutants and controls (in c: ML: p=0.4998, t=0.7652, d.f.=4; GL: p=0.9487, t=0.6992, d.f.=4). Arrowheads (in a) indicate single Iba1+ cells.
Quantitations were performed on parasagittal sections of the cerebellar vermis (n=3 mice each genotype; age 30 weeks). Data are means ± s.e.m. Two-tailed unpaired Student’s t-test. GL, GC layer; ML, molecular layer. Images (in a) are representative of 3 similar experiments.
