Supplementary Figure 12: Conditional inactivation of Pten in principal neurons of the hippocampus
From: A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination
(a,b) Tamoxifen treatment schemes for Nex-CreERT2;Rosa26-lacZ mice (a, top), as used for Cre-mediated activation of lacZ in CA3 pyramidal neurons (image in b is representative of 3 similar experiments.), and for conditional Pten mutants (a, bottom) used for histological analysis (see below).
(c-e) Nex-CreERT2 ;PtenloxP/loxP mutants exhibit the same number of CNP+ oligodendrocytes (arrowheads in c; quantified in d, p=0.8451, t=0.2084, d.f.=4), and a similar (MBP+) myelinated area in the hippocampus (p=0.7849, t=0.2918, d.f.=4) when compared to controls (in e, quantified on coronal sections of the forebrain; n=3 mice each genotype and age). Data are means ± s.e.m. **p<0.01; ***p<0.001, two-tailed unpaired Student’s t-test. CC, corpus callosum; DG, dentate gyrus. Images are representative of 3 similar experiments.
(f-g) Hippocampal CA3 neurons from Nex-CreERT2 ;PtenloxP/loxP mutants are enlarged in size, when quantified in H&E stained paraffin sections (in f, n=3 per genotype; 40 cells per animal, p=0.0005, t=10.18, d.f.=4) and by electron microscopy (in g, n=2 per genotype; 10-15 cells per animal).
Data are means ± s.e.m. ***p<0.001, two-tailed unpaired Student’s t-test. Images are representative of 2 (in g) or 3 (in b,c and f) similar experiments.
