Supplementary Figure 2: Pten mutant mice as a model of Lhermitte-Duclos disease
From: A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination
(a) Gallyas silver impregnation of cerebellar parasagittal sections reveals a focal pathology (asterisks) in aged Pten mutants, preferentially localized to the inferior lobe (IL) and the posterior lobe (PL). Pathology is characterized by secondary focal hypergrowth of GC immunostained for NeuN (arrowheads in b; age 1 year; boxed area magnified on the right), loss of Purkinje cells immunostained for Calbindin (arrowheads in c; age, 1 year; boxed area magnified on the right), loss of central white matter immunostained for CNP (arrowheads in d; age, 1 year; boxed area magnified on the right), and presence of activated microglia cells immunostained for Mac-3 (in e; age, 1.5 years; boxed area magnified on the right) and enhanced vascularization (boxed area in e magnified on the right and further magnified in inset). Cerebellar lobes I-X are marked in c. Images are representative of >3 similar experiments.
