Supplementary Figure 6: Targeted ablation of Pten in a small subset of cerebellar GC
From: A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination
(a) Tamoxifen treatment of Nex-CreERT2;Rosa26-lacZ mutant mice induces Cre-mediated activation of a lacZ reporter gene in a small subset of GC.
(b) Tamoxifen treatment scheme of Nex-CreERT2 ;PtenloxP/loxP mutant mice.
(c-f) Nex-CreERT2 ;PtenloxP/loxP mutants exhibit significantly more CNP+ oligodendrocytes (p< 0.0001, t=19, d.f.=4) and a larger MBP+ area (p=0.009 t=4.739, d.f.=4) in the ML when compared to controls (quantitated in parasagittal sections of the cerebellar vermis; n=3 mice each genotype; age, 30 weeks).
Data are means ± s.e.m. **p<0.01; ***p<0.001, two-tailed unpaired Student’s t-test. GL, GC layer; ML, molecular layer; WM, White matter. Images in a, c and e are representative of 3 similar experiments.
