Supplementary Figure 8: PLD1 and PLD2 expression in the cortex.
From: Astrocytes mediate neurovascular signaling to capillary pericytes but not to arterioles
(a, b) PLD1 was diffusely expressed in the cortical neuropil and appeared concentrated in endothelial cells along arterioles (arrow, a) but not capillaries (b). No PLD1 labeling was observed in AQP4-labeled endfeet (arrowheads). (c, d) PLD2 expression was detected in GFAP-expressing astrocyte endfeet along capillaries (arrowheads, c) as well as in astrocyte somata (asterisks, d). (e) Control experiments where slices were labeled with the secondary antibody used to detect the enzymes shown in the corresponding panels in a-d, but with the primary antibody omitted, demonstrating the lack of non-specific binding. Control for secondary antibodies used to detect PLD1 is shown in the top two panels and for PLD2 is shown in the last two panels (for these pictures illumination was applied appropriate for evoking fluorescence from the secondary antibody used for PLD1/PLD2 labeling and from the dye-conjugated IB4 or DAPI).
