Supplementary Figure 10: Experimental setup for puffing α,β-methylene-ATP.
From: Astrocytes mediate neurovascular signaling to capillary pericytes but not to arterioles
(a) DIC image of slice showing a capillary. (b) DIC image of the slice surface showing position of the puff pipette. (c) Fluorescence image showing the pipette containing Alexa Fluor 594 positioned above the slice. (d-i) The spread of the Alexa Fluor 594 dye imaged at the start (d) and 5 consecutive seconds (e-i) following a 5 s puff at 5 psi. Note how the dye flows downwards and does not reach the location of the vessel at the top (demarcated by white dotted lines).
