Supplementary Figure 4: rAAV-hDlx-Gq-DREADD restricts reporter expression to the membrane of GABAergic interneurons
From: A viral strategy for targeting and manipulating interneurons across vertebrate species
(a) Scheme of the rAAV-hDlx-Gq viral construct. (a-c) Adult Dlx6aCre::RCE (n=4) or (d) C57Bl6 mice (n=16) were injected with rAAV-hDlx-Flex-GFP in somatosensory cortex (S1) or hippocampus (CA1) and were analyzed by immunostaining for the indicated markers after 7 days. Representative example of expression of the reporter dTomato and the indicated marker and corresponding quantifications (S1: 91.9 +/- 0.7%, n= 2091 cells from 4 animals; CA1: 93.9 +/- 2.3%, n= 173 cells from 3 animals). Quantification are indicated in the text as mean ± s.e.m and are represented as box-and-whisker plot with upper and lower whiskers represent the maximum and minimum value respectively and the box represent upper, median and lower quartile. Dashed lines represent limits of the indicated anatomical structures. P – pyramidal layer. Scale bars represent 100μm (a), 25μm (c,d).
