Supplementary Figure 4: Walking direction and speed sensitivity in HS cells

(a) Change in Vm of left-side HS cells with respect to quiescence (ΔVm, color-coded) as a function of the forward and angular velocities of each individual fly. (b) Same as in (a), but for right-side HS cells. Scale bar is the same as for the maps in (a). (c) Population ΔVm (color-coded) as a function of the forward and angular acceleration of the fly. (d) Examples of the Vm of HS cells (orange), the forward (Vf, black), and angular velocities (Va, gray) of the fly in segments of walking bouts where the fly’s angular velocity did not exceed ±35°/s (gray shadow). (e) Left, weighted grand mean±SEM of the average angular velocity of the fly for the selected segments with low angular velocity as a function of the averaged Vf of the fly. The weight is given as a function of number of segments where the fly showed |Va| < 35°/s (mean = 240, range: 50-499 bouts). Right, weighted grand mean±SEM of the average Vm as a function of the average Vf of the fly. (f) Mean correlation coefficient between the fly’s Vf and the Vm of HS cells per cell, and between Vf and Va per cell. Significant correlations are red, non-significant correlations are blue (traces were shuffled 20 times by concatenated walking bouts, Wilcoxon’s signed-rank test).