Supplementary Figure 6: Motion artifact exclusion for imaging sessions with tdTomato structural marker
From: Parallel processing by cortical inhibition enables context-dependent behavior
a, RGB merge image of a PV+ interneuron (green, GCaMP6s; red, tdTomato). b, Green channel image and associated tone-evoked epoch in the active and passive contexts, showing large tone-evoked response. c, Red channel image of same neuron and associated tone-evoked epoch, showing lack of response. The cross correlation of these two signals for this neuron was low (r: 0.2), and so this neuron was included in the analysis. d, Green channel from another example neuron included in the analysis. e, Red channel of the cell in d (cross-correlation coefficient r: 0.4). f, RGB merge image of a PV+ interneuron that was excluded. g, Green channel image and associated tone-evoked epoch in the active and passive contexts. h, Red channel image and associated tone-evoked epoch. Note the high correlation between the red and green channels (r: 0.9) even after bleedthrough correction with linear spectral unmixing.
