Supplementary Figure 14: Histology
From: Parallel processing by cortical inhibition enables context-dependent behavior
a, We confirmed that our optical suppression of PV+ neurons was selective (PV-cre mice injected with AAV1.EF1a.DIO.eNpHR3.0) by co-staining neurons using an anti-PV antibody (red) and one for GFP to recognize the eNpHR3.0-YFP (green). DAPI nuclear staining is in blue. b, Similar co-staining for somatostatin in SOM-cre mice injected with AAV1.EF1a.DIO.eNpHR3.0. c, CaMK2-GCaMP6f is largely excluded from PV+ interneurons, the largest inhibitory subtype in the cortex (93.3±3.3% of GCaMP6+ neurons do not exhibit detectable staining for PV, n=70 GCaMP6+ neurons in 2 mice).
