Supplementary Figure 1: Trial structure for go/no-go behavior

a, Overall timeline of experiments. Day 1: A1 mapping, injection of AAV1-SYN-GCAMP6s, cranial window and headpost implantation. Water restriction typically occurred ~7-10 days before initial training. Day 14-21: baseline imaging session to select the target and foil tones. Days 15-30: animal training. Day 20+: imaging session in trained animals. Variability in timeline in terms of start dates for each phase was largely attributable to GCaMP6s expression timing and behavioral learning trajectory. b, Normalized lick rate for all animals in all sessions in Figure 1, for hit correct trials to the target tone. Thin lines, individual sessions (n=5 mice, 11 sessions); thick line, average performance. c, Normalized lick rate for foil tones (correct reject trials). d, Performance on go/no-go task over sessions for 11 animals (d’ = z(hit rate) – z(false alarm rate). Dashed line indicates d’ of 1.75, criterion for considering animals fully trained on the task.