Supplementary Figure 2: Identification of false positives due to lick-related brain motion

a, Histogram of normalized tone-evoked response for one example mouse (animal Ms22). Lick-related brain motion should produce both positive and negative deflections in fluorescence. We calculated the nominal false positive rate as the percent of negative deflections greater than 5%. Vertical dashed line indicates false positive rate. Each animal used in this study had nominal false positive rates below 5% (i.e., 95% likely that positive deflections are not due to licking). b, Histogram of normalized responses for another mouse (animal Ms31). c, Scatter plot of the nominal false positive rate for all sites in all animals from Figure 2a-d. Orange dots are example sites in a, b. d, Confidence level (positive-negative deflection rate) at each normalized dF value. Even for small changes in fluorescence (<0.05), the probability of lick-related brain motion driving the signals is only <15%. Orange dots are example sites in a, b.