Supplementary Figure 7: CRACM mapping to evaluate ARC →; PVH connectivity.
From: A rapidly acting glutamatergic ARC→PVH satiety circuit postsynaptically regulated by α-MSH
a, Left, Cre-independent ChR2 fused to a fluorescent protein (AAV-ChR2-mCherry) was unilaterally targeted to ARC neurons. Images show expression of AAV-ChR2-mCherry in the injection site and terminal field in the PVH. PVHMC4R neurons were transduced with AAV-DIO-GFP. Scale bars represent 100 μm. Right, schematic shows connections being tested (top) and representative traces of light-evoked EPSCs recorded from a PVHMC4R neuron before (black) and after (grey) bath application of the AMPAR antagonist CNQX. ARC, ARCGlutamatergic. b, c, Top, schematics show connections being tested. Bottom, representative traces showing assessment of light-evoked EPSCs recorded from PVHMC4R-negative (MC4R-) neurons.
