Supplementary Figure 1: Piezo2-Cre;Ai9 expression in P2 mouse brain
From: Mechanosensory hair cells express two molecularly distinct mechanotransduction channels
Piezo2 is not expressed in neurons of the auditory hindbrain and is restricted to endothelial cells, trigeminal nerve fibers, neuropil of trigeminal nuclei and the dorsal horn of the rostral spinal cord. All images are from flattened projections of confocal images taken from sagittal sections at various mediolateral locations of P2 Piezo2-Cre; Ai9 (red) counterstained for DAPI, a nuclear marker (blue). (A) Piezo2 is expressed only by endothelial cells in P2 cerebral cortex and hippocampus. (B) Piezo2 is expressed by endothelial cells in the P2 cerebellum and the choroid plexus of the fourth ventricle but not by any other cell types. (C) Piezo2 is expressed by endothelial cells but no other cell types in the cochlear nucleus nor fibers contacting the cochlear nucleus. Note the labeled trigeminal nerve fibers. (D) The superior olivary nucleus, the major source of olivocochlear efferent neurons, does not express Piezo2, except for endothelial cells. No fibers contacting the superior olivary nucleus express Piezo2. (E) The inferior colliculus, cerebellum and cochlear nuclei do not express Piezo2, except for endothelial cells. Piezo2 is expressed in the trigeminal nerve, trigeminal nuclei, and dorsal horn of the rostral spinal cord. Scale bar in (E) = 500 μm and applies to all images.
