Supplementary Figure 6: Activation of HSD2 neurons does not alter water intake.

Water intake (4 h) expressed as a percentage of baseline intake from two-bottle choice assays in response to CNO (1 mg/kg) administration or the following night (Recovery). (a, b) Water intake in male mice from taste assays in Fig. 2b or paired with food intake from Fig. 2d (two-way ANOVA. CNO Stim, P = 0.6930. Recovery, P = 0.3293) Water intake is not shown from the sucrose taste test because mice predominantly drank sucrose instead of water, and water was not measured during the 3-bottle assay in Fig. 2c. (c, d) Water intake in female mice during the two-bottle choice tests shown in Fig. 1c, or paired with food intake from Fig. 2d (two-way ANOVA. CNO Stim, P = 0.1663. Recovery, P = 0.2376).