Supplementary Figure 9: AAV2-mediated targeting of new neurons in P0-Gi and P42-Gi mice in the olfactory bulbs.

(a) Low power micrographs illustrating medial to lateral distribution of differentiated neurons in the OB generated and transduced with AAV2-Gi (EGFP+, false-colored black) in P0-Gi and P42-Gi mice. Scale bar, 500 μm. (b) Distribution of neurons in the dorsal and ventral domains of the OB, as well as the accessory olfactory bulb (AOB). Scale bar, 50 μm. (c) Dot plots are percentages of EGFP⁺ neurons distributed in distinct regions of the OB calculated as a fraction of the entire EGFP⁺ population of cells counted in the forebrain. Lines are mean ± s.e.m; n=3 mice/group. Layers of the OB: periglomerular (PGL), external plexiform (EPL), mitral (ML), internal plexiform (IPL), superficial granule (GCLs), deep granule (GCLd). Note, while the vast majority of EGFP⁺ neurons in the OB of P42-Gi mice were situated in GCLs and GCLd, P0-Gi mice contained broadly distributed neurons in nearly all olfactory related nuclei, the AOB, and cell layers in the dorsal and ventral aspects of the OB.