Supplementary Figure 4: CNO administration has no effect on responses to an aversive odor in naive Nestin-cre mice.
From: Developmentally defined forebrain circuits regulate appetitive and aversive olfactory learning
(a) Flowchart illustrating strategy to test the effects of CNO administration on responses to an aversive odor (TMT) in P70 Nestin-cre mice. (b) Bar chart illustrates no significant difference in proportions of Freezing and No Freezing responses in vehicle- and CNO-administered control mice over seven days (Fisher’s Exact Test; Number of Freezing trials, vehicle, 35/42; CNO, 36/42; P=0.99; n=6 animals/group). (c) Temporal plot of responses by all vehicle and CNO treated Nestin-cre mice within the 10 minute test period (left y-axis) over the seven days (x-axis). FT, failed trial. Dotted lines represent trendlines calculated from proportions of failed trials (right y-axis) over seven days (shaded regions represent 95% confidence intervals, CI). Significance was tested by Hypothesis test of Linear Regression [Slope (vehicle): 8.93% ± 2.59%/day, CI: 3.7, 14.15; slope (CNO): 7.74% ± 2.48%/day, CI: 2.72, 12.75; H0: slope (vehicle) = slope (CNO); F(1,80)=0.11, P=0.74.]. Fisher’s Exact Test was used to compare proportions of successful trials each day (days 1 to 7 in order: P=1.0, 1.0, 1.0, 1.0, 1.0, 1.0, 1.0).
