Supplementary Figure 5: Non silenced mechanoreceptors are functional after OB-1 treatment.

(a) The proportion of each class of mechanosensitive afferents recorded three hours after local OB-1 or vehicle application is displayed. After OB-1 treatment no significant differences were observed in the receptor proportions among Aβ-, Aδ- or C-fibers compared to vehicle treated skin. Fisher's exact test: Aβ-fibers OB-1 vs. vehicle, P = 0.1722; Aδ-fibers OB-1 vs. vehicle, P = 0.8199; C-fibers OB-1 vs. vehicle, P = 1.0000; numbers indicate single sensory fiber recordings derived from more than 10 independent experiments using adult mice; data are displayed as percentage of individual fibers. (b-e) Receptive field properties of single sensory afferents recorded from vehicle-treated or OB-1 treated skin are shown. (b-d) A series of ramp and hold stimuli with increasing velocities (0.075, 0.15, 0.45, 1.5 and 15 mm/s at 92 μm displacements) was applied to low threshold mechanoreceptors, i.e. slowly adapting mechanoreceptors (SAM) (b), rapidly adapting mechanoreceptors (RAM) (c) and D-hair receptors (d). Mean firing frequencies during the ramp phase are plotted as function of stimulus velocity; numbers indicate fibers recorded; data are displayed as mean number of action potentials ± s.e.m.. (e) An ascending series of displacement stimuli (32 – 1024 μm) using a constant stimulus velocity was applied to A-mechanonociceptors (A-M). Mean firing frequencies were plotted as function of displacement amplitudes showing no changes in mechanosenitivity in A-Ms in OB-1 treated compared to vehicle-treated skin. Two-way ANOVA: SAM vehicle vs. OB-1, P = 0.8924; RAM vehicle vs. OB-1, P = 0.565; D-hair vehicle vs. OB-1, P = 0.8437; A-M vehicle vs. OB-1, P = 0.0912; numbers indicate single sensory fiber recordings derived from more than 5 independent experiments using adult mice; data are displayed as mean number of action potentials ± s.e.m..