Supplementary Figure 1: Effects of STOML3-modulating molecules on other stomatin-domain proteins | Nature Neuroscience

Supplementary Figure 1: Effects of STOML3-modulating molecules on other stomatin-domain proteins

From: Small-molecule inhibition of STOML3 oligomerization reverses pathological mechanical hypersensitivity

Supplementary Figure 1

(a) BiFC signal development observed when cells were transfected with stomatin –VC/-VN, STOML1-VC/-VN, STOML2-VC/-VN, STOML3-VC/-VN or podocin-VC/-VN expression constructs respectively. The slope of signal development was used as a measure of oligomerization. Note that OB-1 significantly inhibits oligomerization of stomatin, STOML1, STOML2 and STOML3 but does not affect podocin oligomerization. Unpaired t-test: stomatin vs. stomatin + OB-1, P < 0.0001; STOML1 vs. STOML1 + OB-1, P < 0.0001; STOML2 vs. STOML2 + OB-1, P < 0.0001; STOML3 vs. STOML3+ OB-1, P < 0.0001; Mann-Whitney U-test: podocin vs. podocin + OB-1, P = 0.0584; numbers indicate replicates derived from 2 independent cell transfections; data are shown as individual slopes and mean ± s.e.m.. (b) OB-1 was tested at two different concentrations (2 μM and 20 μM) using the BiFC assay. The normalized slope was significantly reduced when OB-1 was present. Unpaired t-test: vehicle vs. 20 μm OB-1, P < 0.0001; vehicle vs. 2 μm OB-1, P < 0.0001; numbers indicate replicates derived from two independent cell transfections; data are displayed as individual slopes and mean ± s.e.m. *** P < 0.001; ns nonsignificant.

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