Supplementary Figure 5: GABA_B-receptor- and A₁-receptor-mediated presynaptic inhibition of excitatory synaptic transmission in PV interneurons are not affected by NL3 deletion

a-d, The depression of excitatory synaptic transmission in PV interneurons by the GABA_B receptor agonist baclofen (10 μM) is not affected by NL3 deletion. Time course of normalized AMPAR EPSCs during application of baclofen (a) and sample EPSCs in response to paired pulse stimulation before and after baclofen application (b) in PV-Cre (before-black, after-blue) and NL3^fl/PV-Cre (before-orange, after-green) neurons. Summary plot of normalized EPSC changes due to baclofen (c) and normalized PPR changes (d) in individual cells (PV-Cre, 0.4 ± 0.1, 1.3 ± 0.1, n = 4; NL3^fl/PV-Cre, 0.4 ± 0.1, 1.3 ± 0.04, n = 6) e-h, Same as a-d but for the application of A₁-R agonist N⁶-CPA (1 μM). Representative EPSCs (f) before and after N⁶-CPA for PV-Cre (before-black, after-blue) and NL3^fl/PV-Cre (before-orange, after-green). Individual normalized EPSC (g) and PPR (h) changes caused after N⁶-CPA (PV-Cre, 0.3 ± 0.05, 1.1 ± 0.1, n = 6; NL3^fl/PV-Cre, 0.3 ± 0.03, 1.0 ± 0.02, n = 4).