Supplementary Figure 9: Rescue of synaptic changes in NL3^fl/PV-Cre cells by selective re-expression of NL3.

a, Representative traces of AMPAR EPSCs recorded at -60 mV and dual component EPSC at +40 mV (left). NMDAR/AMPAR ratio (right) is rescued by expression of NL3 in NL3fl/PV-Cre cells (NL3^fl/PV-Cre+Rescue, 0.17 ± 0.02, n = 8 cells; p > 0.1 vs PV-Cre, *p < 0.05 vs NL3fl/PV-Cre). (PV-Cre and NL3fl/PV-Cre data from Fig. 1g.) b, Representative average traces (left) and summary graph (right) show rescue of paired-pulse ratios comparable to PV-Cre cells in NL3^fl/PV-Cre+Rescue cells at inter-stimulus intervals of 20, 50, 100 and 200 ms (PV-Cre, 2.5 ± 0.1, 2.2 ± 0.1, 1.6 ± 0.1, 1.4 ± 0.1, n = 11 cells; NL3^fl/PV-Cre + Rescue, 2.13 ± 0.17, 1.9 ± 0.17, 1.6 ± 0.1, 1.3 ± 0.1, n = 8 cells; p > 0.05 rescue vs control for all ISI). (PV-Cre and NL3fl/PV-Cre data from Fig 2b.) c-e, Inhibition of excitatory synaptic transmission by the Gr-III mGluR agonist L-AP4 is rescued by the expression of NL3 in NL3^fl/PV-Cre cells. c, (left) time course of normalized AMPAR EPSCs during application of L-AP4 and sample EPSCs in response to paired-pulse stimulation before and after L-AP4 application (right) in PV-Cre (before-black, after-blue), NL3^fl/PV-Cre (before-orange, after-green) and NL3^fl/PV-Cre-DIO-NL3 (rescue: before-brown, after-green). d, Normalized summary plot (i) and EPSC amplitudes (ii) (NL3^fl/PV-Cre-DIO-NL3: 0.58 ± 0.1, n = 6, p > 0.1), e, Normalized PPR changes (NL3^fl/PV-Cre-DIO-NL3: 1.3 ± 0.1, n = 7, p > 0.1 vs PV-Cre) (i) and PPR (ii) values in response to L-AP4 application in NL3^fl/PV-Cre-DIO-NL3 cells. (PV-Cre and NL3^fl/PV-Cre data from Fig. 3i-l.)